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Image Search Results
Journal: Allergy, Asthma & Immunology Research
Article Title: The Role of Macrophage Migration Inhibitory Factor (MIF) in Asthmatic Airway Remodeling
doi: 10.4168/aair.2021.13.1.88
Figure Lengend Snippet: MIF, migration inhibitory factor; ASMC, airway smooth muscle cell; TEM, transmission electron microscope; OVA, ovalbumin; ISO-1, (S,R)3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester; DAPI, 4′,6-diamidino-2-phenylindole. * P < 0.05 vs. MIF 0 ng/mL; † P < 0.001 vs. MIF 0 ng/mL; ‡ P < 0.001 vs. control; § P < 0.001 vs. the OVA group.
Article Snippet: The
Techniques: Migration, Transmission Assay, Microscopy, Control
Journal: Allergy, Asthma & Immunology Research
Article Title: The Role of Macrophage Migration Inhibitory Factor (MIF) in Asthmatic Airway Remodeling
doi: 10.4168/aair.2021.13.1.88
Figure Lengend Snippet: MIF, migration inhibitory factor; ASMC, airway smooth muscle cell; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; NC, negative control; ISO-1, (S,R)3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester; q-PCR, quantitative polymerase chain reaction; shRNA, short hairpin RNA. * P < 0.001 vs. the control group; † P < 0.01 vs. the IL-4 group; ‡ P < 0.001 vs. the IL-4 group; § P < 0.01 vs. the IL-4+shRNA-NC group.
Article Snippet: The
Techniques: Migration, Enzyme-linked Immunosorbent Assay, Negative Control, Real-time Polymerase Chain Reaction, shRNA, Control
Journal: Allergy, Asthma & Immunology Research
Article Title: The Role of Macrophage Migration Inhibitory Factor (MIF) in Asthmatic Airway Remodeling
doi: 10.4168/aair.2021.13.1.88
Figure Lengend Snippet: The promotive effects of macrophage-derived MIF on ASMC autophagy determined by immunofluorescence. (A) In the ISO-1-mediated MIF inhibition model, the images of GFP-RFP-LC3-transfected cells showed that ASMC autophagy was decreased in the IL-4+ISO-1 group compared with the IL-4 group (200 ×, scale bar, 10 μm). (B) In the MIF-knock-down model, the IL-4+shRNA-MIF group displayed decreased ASMC autophagy (GFP-RFP-LC3 reporting system) compared with the IL-4 or IL-4+shRNA-NC group (200 ×, scale bar, 10 μm).
Article Snippet: The
Techniques: Derivative Assay, Immunofluorescence, Inhibition, Transfection, Knockdown, shRNA